| DATE | TOPIC | TEXT ASSIGNMENT | LABORATORY EXERCISE |
| Jan 6-10 | Introduction to cellular organization, biomolecules, and a review of the structure and chemistry of water as it applies to living organisms; Amino Acids. | Stryer:Ch1,2
Boyer:29-38 |
Ros 008 Computer Workshop: Use of new lab for data analysis and Molecular Modeling. | |||
| Jan 13-17 | Amino acids and protein chemistry: Primary, Secondary and Tertiary Structure. Purification and Characterization of Protein Structure | Stryer:Ch. 2(cont),3,16 | Molecular Modeling of the Peptide Bond. Determining energy minima and conformational space. | |||
| Jan 20-24 | Protein conformation continued and the oxygen transporters. | Stryer:Ch.7, 16(cont)
Boyer:49-51,90-102, 147-161 |
Laboratory Safety, Chemical properties of amino acids | |||
| Jan 27-31 *Exam | Enzyme kinetics and Mechanisms of catalysis | Stryer:Ch. 8 | Sequence analysis of angiotensin | |||
| Feb3-7 | Mechanisms of Catalysis(cont) | Stryer:Ch. 9
Boyer:191-203,243-250 |
Isolation of rabbit muscle Glyceraldehyde-phosphate dehy-drogenase(GPDH) | |||
| Feb 10-14 | Carbohydrate structure and chemistry, Introduction to metabolism, Glycolysis | Stryer:Ch.18 | Enzyme assays of GPDH | |||
| Feb 17-21 | Glycolysis and bioenergetics | Stryer:Ch 17,19
Boyer:51-56 |
Quantitative methods of protein det. for GPDH | |||
| Feb 24-28 | Pentose Phosphate Pathway, gluconeogenisis, and glycogen metabolism, | Stryer:Ch. 22, 23 Boyer:115-145 | SDS Gel electro-phoresis analysis of GPDH | |||
| Mar 3-7
*Exam |
Structure of Lipids and Biological Membranes, Citric Acid Cycle; kinetic regulation of metabolic processes | Stryer:Ch. 11,20
Boyer:61-65,323-334 |
Isolation of membrane lipids from E. coli | |||
| Mar 11-14 | Electron Transport and Oxidative phosphorylation. Review for Final Exam | Stryer:Ch. 21 Boyer:65-70,335-346 | Preparative TLC and GC analysis of Fatty Acid content in membrane lipids |